Conventional and nested PCR primers
Target Organism | Forward primer sequence (5՛-3՛) | Reverse primer sequence (5՛-3՛) | Target | Accession number of target sequence | |
---|---|---|---|---|---|
First primer | GGAGAGGGAGCCTGAGAGAT | ACTTGCCCTCCAATTGTTTATCG | 18s rRNA | KX908213.1 | |
Second primer | GAGATGGCTACCACATCCAA | GGATTGGGTAATTTACGCGC | |||
First primer |
TGTGTTCAATATCTCCCTGCAAA | GCATGTCGATTCTAATTCAGCTG | Cowp1 | AB089292.1 | |
Second primer | TGTGTTCAATATCTCCCTGCA | CTGGTGCCATACATTGTTGT | |||
First primer |
GAGGTCAAGAAGTCCGCCG | CAAGGGACTTGCGGAAGTTT | betagiardin | XM_001705373.1 | |
Second primer | CGCCGACAACATGTACCT | CATGGTGTCGATCTCCTCC | |||
First primer |
GCGGACGGCTCATTATAACA | TGTCGTGGCATCCTAACTCA | 18s rRNA | X65163.1 | |
Second primer | CGGACGGCTCATTATAACAG | ACAAACTGGATCGTCTCAAG | |||
First primer |
TTAGACCTTAGACAACGGATGTCTTG | TGTGCATTCAAAGATCGAACTTATC | 18s rRNA | JQ677163.1 | |
Second primer | CAACGGATGTCTTGGCTCT | TGCAACGTTCTTCATCGTG |
*The first primers of the parasites are from Won et al. [2].