LMO

Table. 1.

Conventional and nested PCR primers

Target Organism Forward primer sequence (5՛-3՛) Reverse primer sequence (5՛-3՛) Target Accession number of target sequence
Blastocystis hominis First primer GGAGAGGGAGCCTGAGAGAT ACTTGCCCTCCAATTGTTTATCG 18s rRNA KX908213.1
Second primer GAGATGGCTACCACATCCAA GGATTGGGTAATTTACGCGC
Cryptosporidium parvum First primer* TGTGTTCAATATCTCCCTGCAAA GCATGTCGATTCTAATTCAGCTG Cowp1 AB089292.1
Second primer TGTGTTCAATATCTCCCTGCA CTGGTGCCATACATTGTTGT
Giardia lamblia First primer* GAGGTCAAGAAGTCCGCCG CAAGGGACTTGCGGAAGTTT betagiardin XM_001705373.1
Second primer CGCCGACAACATGTACCT CATGGTGTCGATCTCCTCC
Entamoeba histolytica First primer* GCGGACGGCTCATTATAACA TGTCGTGGCATCCTAACTCA 18s rRNA X65163.1
Second primer CGGACGGCTCATTATAACAG ACAAACTGGATCGTCTCAAG
Dientamoeba fragilis First primer* TTAGACCTTAGACAACGGATGTCTTG TGTGCATTCAAAGATCGAACTTATC 18s rRNA JQ677163.1
Second primer CAACGGATGTCTTGGCTCT TGCAACGTTCTTCATCGTG

*The first primers of the parasites are from Won et al. [2].

Lab Med Online 2020;10:221~226 https://doi.org/10.3343/lmo.2020.10.3.221
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