LMO

Table. 2.

Comparison of two real-time PCR, conventional PCR, and nested PCR results

Sample No. EPP AGPA Conventional PCR Nested PCR
3 Negative BH BH BH
5 Negative BH BH BH
11 Negative GL BH BH
15 Negative BH BH BH
19 Negative BH BH BH
22 Negative GL Negative Negative
23 Negative BH BH BH
32 Negative BH, DF BH BH
38 Negative BH BH BH
42 Negative BH BH BH
65 Negative BH BH BH
77 Negative BH BH BH
89 Negative BH BH BH
90 Negative BH BH BH
91 Negative BH BH BH
97 Negative BH BH BH
101 EH EH EH EH
115 Negative BH BH BH
118 Negative BH BH BH
163 Negative GL, EH, CR Negative Negative
220 EH EH EH EH
238 Negative BH BH BH
268 Negative BH BH BH
269 Negative BH Negative BH

Abbreviations: EPP, BD MAXTM Enteric Parasite Panel; AGPA, AllplexTM Gastrointestinal Parasite Assay; PCR, polymerase chain reaction; EH, Entamoeba histolytica; BH, Blastocystis hominis; GL, Giardia lamblia; CR, Cryptosporidium parvum/hominis; DF, Dientamoeba fragilis.

Lab Med Online 2020;10:221~226 https://doi.org/10.3343/lmo.2020.10.3.221
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