Laboratory Medicine Online

Table. 3. Table 3

Example of sample selection for a comparison of results between a new method and a method already established in the laboratory

Reference	Test	Sample selection	Number of samples	Comparison method
Chung et al. [15]	Quantification of BCR-ABL1 fusion transcript using droplet digital PCR	Clinical samples ranging from 0.002% IS [MR4.7] to 20% IS [MR0.7]	20	Real-time PCR
Yuan et al. [21]	Quantification of PML-RARA fusion transcript using droplet digital PCR	Clinical samples including from healthy individuals, newly diagnosed patients, and treated patients	28	Real-time PCR
Wolstencroft et al. [22]	JAK2 V617F mutation using real-time PCR	Clinical samples from patients referred for investi- gation of myeloproliferative disorders	200	ARMS and allele-specific PCR
Schnittger et al. [23]	BRAF V600E mutation using real-time PCR	Clinical samples from patients at diagnosis of hairy cell leukemia	117	Multiparameter flow cytometry

Abbreviations: IS, international scale; MR, molecular response; PCR, polymerase chain reaction; ARMS, amplification refractory mutation system.