Table. 4.
Minimum starting material requirements for achieving a low limit of detection in ddPCR [20]
| LOD | Required starting material | Number of droplets | Number of wells | ||
|---|---|---|---|---|---|
| Total copies | Diploid cells | Amount of DNA | |||
| 1 in 1,000 | 3,000 | 1,500 | 0.010 µg | 1,500 | 1 |
| 1 in 10,000 | 30,000 | 15,000 | 0.10 µg | 15,000 | 1 |
| 1 in 100,000 | 300,000 | 150,000 | 1.0 µg | 150,000 | 8 |
Abbreviation: LOD, limit of detection.
Lab Med Online 2022;12:227~234 https://doi.org/10.47429/lmo.2022.12.4.227
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