Table. 5.
Strategy for an empirical determination of the LOD of ddPCR in a single well
| Number of wells | Purpose | DNA content |
|---|---|---|
| 1 NTC | Monitor contamination | No DNA |
| 2-3 wild type only control | Monitor LOB | Wild type only genomic DNA per 130 ng/reaction |
| 4-5 serial dilutions of MPC | Monitor LOD | Serial dilution of mutant spiked into 130 ng/reaction of wild type genomic DNA making the concentration of mutant DNA near suggested LOD |
Abbreviations: LOD, limit of detection; NTC, no-template control; LOB, limit of blank; MPC, mutation-positive control.
Lab Med Online 2022;12:227~234 https://doi.org/10.47429/lmo.2022.12.4.227
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