Table. 1.
Gating strategy for detecting lymphoid subpopulations using PIDOT panel
| Population | Gating strategy |
|---|---|
| B-cells | CD45hi/CD16-CD56-/CD3-/CD19+ |
| Pre-GC B-cells | CD27-/smIgM+/smIgD+ |
| Post-GC B-cells | |
| Unswitched MBC/PC | CD27+/smIgM+/smIgD+ |
| Switched MBC/PC | CD27+/smIgM-/smIgD- |
| T-cells | CD45hi/CD16-CD56-/CD3+/CD19- |
| CD4+ T-cells | CD4+/CD8-/TCRγδ- |
| Naïve CD4+ T-cells | CD27+/CD45RA+ |
| CM/ TM CD4+ T-cells | CD27+/CD45RA- |
| EM CD4+ T-cells | CD27-/CD45RA- |
| TD CD4+ T-cells | CD27-/CD45RA+ |
| CD8+ T-cells | CD4-/CD8+/ TCRγδ- |
| Naïve CD8+ T-cells | CD27+/CD45RA+ |
| CM/TM CD8+ T-cells | CD27+/CD45RA- |
| Effector CD27dim CD8+ T-cells | CD27int/CD45RA+ |
| EM CD8+ T-cells | CD27-/CD45RA- |
| TD CD8+ T-cells | CD27-/CD45RA+ |
| NK cells | CD45hi/CD16+CD56+/CD3-/CD19- |
Abbreviations: PIDOT, Primary Immunodeficiency Orientation Tube; CD, cluster of differentiation; CM, central memory; EM, effector memory; GC, germinal center; MBC/PC, memory B-cells/plasma cells; NK, natural killer; smIg, surface membrane immunoglobulin; TD, terminally differentiated; TM, transitional memory.
Lab Med Online 2023;13:53~59 https://doi.org/10.47429/lmo.2023.13.1.53
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