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Table. 1.

Table. 1.

Gating strategy for detecting lymphoid subpopulations using PIDOT panel

Population Gating strategy
B-cells CD45hi/CD16-CD56-/CD3-/CD19+
Pre-GC B-cells CD27-/smIgM+/smIgD+
Post-GC B-cells
Unswitched MBC/PC CD27+/smIgM+/smIgD+
Switched MBC/PC CD27+/smIgM-/smIgD-
T-cells CD45hi/CD16-CD56-/CD3+/CD19-
CD4+ T-cells CD4+/CD8-/TCRγδ-
Naïve CD4+ T-cells CD27+/CD45RA+
CM/ TM CD4+ T-cells CD27+/CD45RA-
EM CD4+ T-cells CD27-/CD45RA-
TD CD4+ T-cells CD27-/CD45RA+
CD8+ T-cells CD4-/CD8+/ TCRγδ-
Naïve CD8+ T-cells CD27+/CD45RA+
CM/TM CD8+ T-cells CD27+/CD45RA-
Effector CD27dim CD8+ T-cells CD27int/CD45RA+
EM CD8+ T-cells CD27-/CD45RA-
TD CD8+ T-cells CD27-/CD45RA+
NK cells CD45hi/CD16+CD56+/CD3-/CD19-

Abbreviations: PIDOT, Primary Immunodeficiency Orientation Tube; CD, cluster of differentiation; CM, central memory; EM, effector memory; GC, germinal center; MBC/PC, memory B-cells/plasma cells; NK, natural killer; smIg, surface membrane immunoglobulin; TD, terminally differentiated; TM, transitional memory.

Lab Med Online 2023;13:53~59 https://doi.org/10.47429/lmo.2023.13.1.53
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