Table. 2.
Quality control metrics (A CytoScan™ Dx example)
| Quality control metrix | Method | Acceptable limit | |
|---|---|---|---|
| Wet bench process | DNA yield after extraction | Fluorometer, sphectrophotometer | ≥ 50 ng/μL or total ≥ 250 ng |
| DNA yield after PCR | Fluorometer, sphectrophotometer | ≥ 3 μg/μL | |
| DNA purity | Optical density ratio, A260/A280 | 1.7-2.1 | |
| DNA size after PCR | Gel electrophoresis (2%) | 150-2,000 bp | |
| DNA size after fragmentation | Gel electrophoresis (4%) | 25-125 bp | |
| Bioinformatic process | Median absolute pairwise difference (MAPD) | Software | ≤ 0.25 |
| SNP QC | Software | ≥ 12 | |
| Waviness SD | Software | ≤ 0.12 |
Lab Med Online 2023;13:141~153 https://doi.org/10.47429/lmo.2023.13.3.141
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