Comparison of total and hands-on times using manual vs. automated sample preparation methods used in this study for 48 samples
Protocol steps | Manual | Automated |
---|---|---|
Sample transfer | ||
Calibrator (N = 6), QC (N = 3), blank (N = 2), and sample (N = 39) transfer | 6 min | 27 min |
Protein precipitation | ||
0.1 M ZnSO4 addition (and mixing) | 8 min | 2 min |
Internal standard solution addition (and mixing) | 10 min | 3 min |
Centrifugation | 10 min | 20 min |
Sample Transfer | ||
Supernatant transfer | 3 min | 3 min |
Total time | 37 min | 55 min |
Total hands-on time | 27 min | 2 min |