Table. 2.
Analytical imprecision of the Andrew+-based liquid chromatography/tandem mass spectrometry assay
| Analyte | QC Level | Mean (ng/mL) | Repeatability |
Within-laboratory Precision |
||
|---|---|---|---|---|---|---|
| SD (ng/mL) | CV% | SD (ng/mL) | CV% | |||
| Tacrolimus | Level 1 | 2.6 | 0.18 | 7.1 | 0.24 | 9.2 |
| Level 2 | 7.0 | 0.31 | 4.4 | 0.41 | 5.8 | |
| Level 3 | 14.8 | 0.51 | 3.5 | 0.59 | 4.0 | |
| Sirolimus | Level 1 | 2.8 | 0.26 | 9.6 | 0.27 | 10.0 |
| Level 2 | 10.1 | 0.66 | 6.5 | 0.93 | 9.2 | |
| Level 3 | 19.3 | 1.06 | 5.5 | 1.40 | 7.2 | |
| Everolimus | Level 1 | 2.5 | 0.22 | 8.8 | 0.28 | 11.1 |
| Level 2 | 4.7 | 0.39 | 8.3 | 0.43 | 9.2 | |
| Level 3 | 9.0 | 0.41 | 4.6 | 0.55 | 6.1 | |
| Cyclosporine A | Level 1 | 46.9 | 1.48 | 3.1 | 2.45 | 5.2 |
| Level 2 | 248.3 | 4.24 | 1.7 | 5.37 | 2.2 | |
| Level 3 | 492.5 | 8.91 | 1.8 | 11.48 | 2.3 | |
*Repeatability and †within-laboratory precision were assessed using three QC materials assayed over 5 days, with two runs per day, and five replicates per run: a 5×2×5 design.
Lab Med Online 2023;13:199~204 https://doi.org/10.47429/lmo.2023.13.3.199
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